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DNA Based Method for Differentiation of Cow, Buffalo, Sheep, Goat and Camel Milk

Technology Details:
Cattle produce 83% of world milk production, followed by buffaloes with 13%, goats with 2%, sheep with 1% and camel with 0.3%. The remaining share is produced by other dairy species such as equines and yaks. About one-third of the milk production in developing countries comes from buffaloes, goats, camels and sheep. In developed countries, almost all milk is produced by cattle. This is an innovative process for the isolation of milk somatic cells. The invention relates to a process for purifying and isolating milk somatic cells from raw and packed milk. Subsequently nucleic acids are isolated from the milk somatic cells. The basis of the invention is a method of isolation and disruption of the milk somatic cells, protecting the nucleic acids and finally purifying them. The isolated DNA was processed further for tracking their origin of species by PCR.